Alcohol
Volume 21, Issue 3 , Pages 251-257 , July 2000

Transcriptional induction of bilirubin UDP-glucuronosyltransrase by ethanol in rat liver

  • Tamás Kardon

      Affiliations

    • Department of Medical Chemistry, Semmelweis University of Medicine, P.O. Box 260, H-1444 Budapest, Hungary
    • ,
  • Marcus Jonathan Coffey

      Affiliations

    • Department of Molecular and Cellular Pathology, University of Dundee, Ninewells Hospital and Medical School, Dundee DD1 9SY, UK
    • ,
  • Gábor Bánhegyi

      Affiliations

    • Department of Medical Chemistry, Semmelweis University of Medicine, P.O. Box 260, H-1444 Budapest, Hungary
    • ,
  • Abigail Ann Conley

      Affiliations

    • Department of Molecular and Cellular Pathology, University of Dundee, Ninewells Hospital and Medical School, Dundee DD1 9SY, UK
    • ,
  • Brian Burchell

      Affiliations

    • Department of Molecular and Cellular Pathology, University of Dundee, Ninewells Hospital and Medical School, Dundee DD1 9SY, UK
    • ,
  • József Mandl

      Affiliations

    • Department of Medical Chemistry, Semmelweis University of Medicine, P.O. Box 260, H-1444 Budapest, Hungary
    • Corresponding Author InformationCorresponding author. Tel.: +36-1-266-26-15; fax: +36-1-266-26-15
    • ,
  • László Braun

      Affiliations

    • Department of Medical Chemistry, Semmelweis University of Medicine, P.O. Box 260, H-1444 Budapest, Hungary

Received 7 May 1999 ,Revised 18 January 2000 ,Accepted 10 April 2000.

References 

  1. Bock KW, Burchell B, Dutton GJ, Hanninen O, Mulder OJ, Owens IS, et al. UDP-glucuronosyltransferase activities. Guidelines for consistent interim terminology and assay conditions. Biochem Pharmacol. 1983;32:953
  2. Braun L, Coffey MJ, Puskás F, Kardon T, Nagy G, Conley AA, et al. Molecular basis of bilirubin UDP-glucuronosyltransferase induction in spontaneously diabetic rats, acetone-treated rats and starved rats. Biochem J. 1998;336:587–592
  3. Burchell B. Substrate specificity and properties of uridine diphosphate glucuronosyltransferase purified to apparent homogeneity from phenobarbital-treated rat liver. Biochem J. 1978;173:749–757
  4. Burchell B. Reconstitution of purified Wistar rat liver bilirubin UDP-glucuronyltransferaseinto Gunn-rat liver microsomes. Biochem J. 1982;201:653–656
  5. Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate–phenol–chloroform extraction. Anal Biochem. 1987;162:156–159
  6. Clarke DJ, Burchell B. The uridine diphosphate glucuronosyltransferase multigene family: function and regulation. In:  Kauffman FC editors. Conjugation–Deconjugation Reactions in Drug Metabolism and Toxicity. Budapest: Springer-Verlag; 1994;p. 3–43
  7. Coughtrie MWH, Burchell B, Shepherd IM, Bend JR. Defective induction of phenol glucuronidation by 3-methylcholanthrene in Gunn rats is due to the absence of a specific UDP-glucuronosyltransferase isoenzyme. Mol Pharmacol. 1987;31:585–591
  8. Dioguardi N, Ideo G, Del Ninno E, de Franchis R. Induction of liver UDPGT by ethanol. Lancet. 1970;1(655):1063
  9. Finley BL, Ashley PJ, Neptune AG, Yost GS. Substrate-selective induction of rabbit hepatic UDP-glucuronosyltransferases by ethanol and other xenobiotics. Biochem Pharmacol. 1986;35:2875–2881
  10. Ideo G, de Franchis R, Del Ninno E, Dioguardi N. Ethanol increases liver uridine-diphosphate-glucuronosyltransferase. Experientia. 1971;27:24–25
  11. Koop DR, Tierney DJ. Multiple mechanisms in the regulation of ethanol-inducible cytochrome P450IIE1. BioEssays. 1990;12:429–435
  12. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970;227:680–685
  13. Lieber CS, Rubin E. Alcoholic fatty liver. N Engl J Med. 1969;280:705
  14. Narayan SS, Hayton WL, Yost GS. Chronic ethanol consumption causes increased glucuronidation of morphine in rabbits. Xenobiotica. 1991;21:515–524
  15. Okolicsany L, Cartei G, Naccarato R. Effects of ethanol on Gilbert's hyperbilirubinemia. Lancet. 1972;747:450
  16. Phelan D, Winter GM, Rogers WJ, Lam JC, Denison MS. Activation of the Ah receptor signal transduction pathway by bilirubin and biliverdin. Arch Biochem Biophys. 1998;357:155–163
  17. Rubin E, Lieber CS. Alcohol-induced hepatic injury in nonalcoholic volunteers. N Engl J Med. 1968;278:869
  18. Sambrook J, Fritsch EF, Maniatis T. Molecular Cloning: A Laboratory Manual. 2nd edn.. Cold Spring Harbour, New York: Cold Spring Harbour Laboratory; 1989;
  19. Samson D, Halliday D, Chanarin I. Enhancement of bilirubin clearance and hepatic haem turnover by ethanol. Lancet. 1976;2(7979):256
  20. Sato H, Koiwai O, Tanabe K, Kashiwamata S. Isolation and sequencing of rat liver bilirubin UDP-glucuronosyltransferase cDNA: possible alternate splicing of a common primary transcript. Biochem Biophys Res Commun. 1990;169:260–264
  21. Sieg A, Seitz HK. Increased production, hepatic conjugation, and biliary secretion in the rat after chronic ethanol consumption. Gastroenterology. 1987;93:361–366
  22. Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedures and some applications. Proc Natl Acad Sci USA. 1979;76:4350–4354
  23. Visser J. Importance of deiodination and conjugation in the hepatic metabolism of thyroid hormone. In:  Greer MA editors. The Thyroid Gland. New York: Raven Press; 1990;p. 255–283
  24. Walters MI, Gerarde HW. An ultramicromethod for the determination of conjugated and total bilirubin in serum or plasma. Microchem J. 1970;15:231
  25. Waltman R, Bonura F, Nigrin G, Pipat C. Ethanol in prevention of hyperbilirubinaemia in the newborn. A controlled trial. Lancet. 1969;2(633):1265–1267

PII: S0741-8329(00)00095-1

Alcohol
Volume 21, Issue 3 , Pages 251-257 , July 2000

Article Tools

* Image Usage & Resolution