Alcohol
Volume 24, Issue 3 , Pages 179-187 , July 2001

Impaired immunoglobulin M production by incubation of hybridoma cells with ethanol

  • Eckhard Mühlbauer

      Affiliations

    • Department of Clinical Neurobiology, Benjamin-Franklin School of Medicine, Free University of Berlin, 14050 Berlin, Germany
    • Corresponding Author InformationCorresponding author. Department of Anatomie und Zellbiologie, Martin Luther Universität Halle-Wittenberg, Grosse Steinstrasse 52, D-06097 Halle, Germany. Tel.: +49-0345-557-1711; fax: +49-0345-557-1700
  • ,
  • Uwe Karsten

      Affiliations

    • Max-Delbrück Center for Molecular Medicine,13092 Berlin-Buch, Germany
  • ,
  • Matthias Rottmann

      Affiliations

    • Department of Clinical Neurobiology, Benjamin-Franklin School of Medicine, Free University of Berlin, 14050 Berlin, Germany
  • ,
  • Hans Rommelspacher

      Affiliations

    • Department of Clinical Neurobiology, Benjamin-Franklin School of Medicine, Free University of Berlin, 14050 Berlin, Germany

Received 27 April 2000 ,Revised 2 February 2001 ,Accepted 17 February 2001.

References 

  1. Beggah A, Mathews P, Beguin P, Geering K. Degradation and endoplasmic reticulum retention of unassembled α- and ß-subunits of Na,K-ATPase correlate with interaction of BiP. J Biol Chem. 1996;271:20895–20902
  2. Bodenheimer H, Charland C, Leith J. Alteration of rat Kupffer cell function following mitomycin-C administration. J Leukocyte Biol. 1988;43:265–270
  3. Brewer JW, Corley RB. Quality control in protein biogenesis: thiol-mediated retention monitors the redox state of proteins in the endoplasmic reticulum. J Cell Sci. 1996;109:2383–2392
  4. Brodie C, Domenico J, Mazer BD, Gelfand EW. Ethanol inhibits ligand-activated Ca2+channels in human B lymphocytes. J Cell Physiol. 1992;152:441–447
  5. Catlin MC, Guizzetti M, Costa LG. Effects of ethanol on calcium homeostasis in the nervous system: implications for astrocytes. Mol Neurobiol. 1999;19:1–24
  6. Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidium thiocyanate–phenol–chloroform extraction. Anal Biochem. 1987;162:156–159
  7. Chroneos ZC, Baynes JW, Thorpe SR. Identification of liver endothelial cells as the primary site of IgM catabolism in the rat. Arch Biochem Biophys. 1995;319:63–73
  8. Cook RT. Alcohol abuse, alcoholism, and damage to the immune system: a review. Alcohol Clin Exp Res. 1998;22:1927–1942
  9. Deviere J, Content J, Denys C, Vandenbussche P, Schandene L, Wybran J, et al. High interleukin-6 serum levels and increased production by leucocytes in alcoholic liver cirrhosis: correlation with IgA serum levels and lymphokines production. Clin Exp Immunol. 1989;77:221–225
  10. Diamond I, Messing RO. Neurologic effects of alcoholism. West J Med. 1994;161:279–287
  11. Faunce DE, Gregory MS, Kovacs EJ. Acute ethanol exposure prior to thermal injury results in decreased T-cell responses mediated in part by increased production of IL-6. Shock. 1998;10:135–140
  12. Feinberg AP, Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983;132:6–13
  13. Figliomeni ML, Turkall RM. Developmental immunotoxicity of cocaine and ethanol in postnatal Lewis rats. Immunopharmacology. 1997;36:41–48
  14. Giron JA, Alvarez-Mon M, Menendez-Caro JL, Abreu L, Albillos A, Manzano L, et al. Increased spontaneous and lymphokine-conditioned IgA and IgG synthesis by B cells from alcoholic cirrhotic patients. Hepatology. 1992;16:664–670
  15. Gluud C, Tage-Jensen U. Autoantibodies and immunoglobulins in alcoholic steatosis and cirrhosis. Acta Med Scand. 1983;214:61–66
  16. Gonzalez-Quintela A, Vidal C, Lojo S, Perez LF, Otero-Anton E, Gude F, et al. Serum cytokines and increased total serum IgE in alcoholics. Ann Allergy Asthma Immunol. 1999;83:61–67
  17. Grossmann M, O'Reilly LA, Gugasyan R, Strasser A, Adams JM, Gerondakis S. The anti-apoptotic activities of Rel and RelA required during B-cell maturation involve the regulation of Bcl-2 expression. EMBO J. 2000;19:6351–6360
  18. Haas IG, Wabl M. Immunoglobulin heavy chain binding protein. Nature. 1983;306:387–389
  19. Helm RM, Wheeler G, Burks AW, Hakkak R, Badger TM. Flow cytometric analysis of lymphocytes from rats following chronic ethanol treatment. Alcohol. 1996;13:467–471
  20. Hendershot LM. Immunoglobulin heavy chain and binding protein complexes are dissociated in vivo by light chain addition. J Cell Biol. 1990;111:829–837
  21. Höhfeld J, Jentsch S. GrpE-like regulation of the hsc70 chaperone by the anti-apoptotic protein BAG-1. EMBO J. 1997;16:6209–6216
  22. Ito M, Jameson JL, Ito L. Molecular basis of autosomal dominant neurohypophyseal diabetes insipidus: cellular toxicity caused by the accumulation of mutant vasopressin precursors within the endoplasmic reticulum. J Clin Invest. 1997;99:1897–1905
  23. Karsten U, Butschak G, Cao Y, Goletz S, Hanisch FG. A new monoclonal antibody (A78-G/A7) to the Thomsen–Friedenreich pan-tumor antigen. Hybridoma. 1995;14:37–44
  24. Kawakami M, Meyer AA, deSerres S, Peterson HD. Effects of acute ethanol ingestion and burn injury on serum immunoglobulin. J Burn Care Rehabil. 1990;11:395–399
  25. Laso FJ, Madruga JI, Lopez A, Cuidad J, Alvarez-Mon M, San Miguel J, et al. Abnormalities of peripheral blood T lymphocytes and natural killer cells in alcoholic hepatitis persist after a 3-month withdrawal period. Alcohol Clin Exp Res. 1997;21:672–676
  26. McClain CJ, Barve S, Deaciuc I, Kugelmas M, Hill D. Cytokines in alcoholic liver disease. Semin Liver Dis. 1999;19:205–219
  27. Miles MF, Diaz JE, DeGuzman VS. Mechanisms of neuronal adaptation to ethanol: ethanol induces Hsc70 gene transcription in NG108-15 neuroblastoma xglioma cells. J Biol Chem. 1991;266:2409–2414
  28. Miles MF, Wilke N, Elliot M, Tanner W, Shah S. Ethanol-responsive genes in neural cells include the 78-kilodalton glucose-regulated protein (GRP78) and 94-kilodalton glucose-regulated protein (GRP94) molecular chaperones. Mol Pharmacol. 1994;46:873–879
  29. Mili F, Flanders WD, Boring JR, Annest JL, DeStefano F. The associations of alcohol drinking and drinking cessation to measures of immune system in middle-aged men. Alcohol Clin Exp Res. 1992;16:688–694
  30. Oberdoerster J, Kamer AR, Rabin RA. Differential effect of ethanol on PC12 cell death. J Pharmacol Exp Ther. 1998;287:359–365
  31. Razani-Boroujerdi S, Savage SM, Sopori ML. Alcohol-induced changes in the immune response: immunological effects of chronic ethanol intake are genetically regulated. Toxicol Appl Pharmacol. 1994;127:37–43
  32. Reddy PS, Corley RB. Assembly, sorting, and exit of oligomeric proteins from the endoplasmic reticulum. Biossays. 1998;20:546–554
  33. Reiprich K, Mühlbauer E, Decuypere E, Grossmann R. Characterization of growth hormone gene expression in the pituitary and plasma growth hormone concentrations during posthatch development in the chicken. J Endocrinol. 1995;145:343–353
  34. Rifai A, Fadden K, Morrison SL, Chintalacharuvu KR. The N-glycans determine the differential blood clearance and hepatic uptake of human immunoglobulin (Ig)A1 and IgA2 isotypes. J Exp Med. 2000;191:2171–2182
  35. Roccatello D, Picciotto G, Torchio M, Ropolo R, Ferro M, Franceschini R, et al. Removal systems of immunoglobulin A and immunoglobulin A containing complexes in IgA nephropathy and cirrhosis patients: the role of asialoglycoprotein receptors. Lab Invest. 1993;69:714–723
  36. Seelig L, Steven W, Stewart M. Effects of maternal ethanol consumption on the subsequent development of immunity to Trichinella spiralis in rat neonates. Alcohol Clin Exp Res. 1996;20:514–522
  37. Shachar I, Rabinovich E, Kerem A, Bar-Nun S. Thiol-reducing agents and calcium perturbants alter intracellular sorting of immunoglobulin M. J Biol Chem. 1994;269:27344–27350
  38. Slukvin II, Jerrells TR. Different pathways of in vitro ethanol-induced apoptosis in thymocytes and splenic T and B lymphocytes. Immunopharmacology. 1995;31:43–57
  39. Tabata T, Meyer AA. Immunoglobulin M synthesis after burn injury: the effects of chronic ethanol on postinjury synthesis. J Burn Care Rehabil. 1995;16:400–406
  40. Van de Wiel A, Seifert WF, Van der Linden JA, Gmelig-Meyling FH, Kater L, Schuurman HJ. Spontaneous IgA synthesis by blood mononuclear cells in alcoholic liver disease. Scand J Immunol. 1987;25:181–187
  41. Watson RR, Prabhala RH, Abril E, Smith TL. Changes in lymphocyte subsets and macrophage functions from high, short-term dietary ethanol in C57/BL6 mice. Life Sci. 1988;43:865–870
  42. Williger BT, Ostermann J, Exton JH. Arfaptin 1, an ARF-binding protein, inhibits phospholipase D and endoplasmic reticulum/Golgi protein transport. FEBS Lett. 1999;443:197–200
  43. Winitz D, Shachar I, Elkabetz Y, Amitay R, Samuelov M, Bar-Nun S. Degradation of distinct assembly forms on immunoglobulin M occurs in multiple sites in permeabilized B cells. J Biol Chem. 1996;271:27645–27651

PII: S0741-8329(01)00152-5

doi: 10.1016/S0741-8329(01)00152-5

Alcohol
Volume 24, Issue 3 , Pages 179-187 , July 2001