Alcohol
Volume 27, Issue 3 , Pages 179-183 , July 2002

S-Adenosyl-l-methionine and mitochondrial reduced glutathione depletion in alcoholic liver disease

  • José C Fernández-Checa

      Affiliations

    • Corresponding Author InformationCorresponding author. Liver Unit, Hospital Clinic I Provincial, Villarroel, 170, 08036 Barcelona, Spain. Tel.: +34-93-227-5709; fax: +34-93-451-5272.
    • Liver Unit, Hospital Clinic I Provincial, 08036 Barcelona, Spain
    • Instituto Investigaciones Biomedicas de Barcelona, IDIBAPS, 08036 Barcelona, Spain
    • Consejo Superior Investigaciones Cientificas, 08036 Barcelona, Spain
    • ,
  • Anna Colell

      Affiliations

    • Liver Unit, Hospital Clinic I Provincial, 08036 Barcelona, Spain
    • Instituto Investigaciones Biomedicas de Barcelona, IDIBAPS, 08036 Barcelona, Spain
    • Consejo Superior Investigaciones Cientificas, 08036 Barcelona, Spain
    • ,
  • Carmen Garcı́a-Ruiz

      Affiliations

    • Liver Unit, Hospital Clinic I Provincial, 08036 Barcelona, Spain
    • Instituto Investigaciones Biomedicas de Barcelona, IDIBAPS, 08036 Barcelona, Spain
    • Consejo Superior Investigaciones Cientificas, 08036 Barcelona, Spain

Received 4 February 2002 ,Revised 28 March 2002 ,Accepted 28 March 2002.

References 

  1. Colell A, Coll O, Garcı́a-Ruiz C, Paris R, Tiribelli C, Kaplowitz N, et al. Tauroursodeoxycholic acid protects hepatocytes from ethanol-fed rats against tumor necrosis factor–induced cell death by replenishing mitochondrial glutathione. Hepatology. 2001;34:964–971
  2. Colell A, Garcı́a-Ruiz C, Miranda M, Ardite E, Mari M, Morales A, et al. Selective glutathione depletion of mitochondria by ethanol sensitizes hepatocytes to tumor necrosis factor. Gastroenterology. 1998;115:1541–1551
  3. Colell A, Garcı́a-Ruiz C, Morales A, Ballesta A, Ookhtens M, Rodes J, et al. Transport of reduced glutathione in hepatic mitochondria and mitoplasts from ethanol-treated rats (effect of membrane physical properties and S-adenosyl-l-methionine). Hepatology. 1997;26:699–708
  4. Corrales F, Gimenez A, Alvarez L, Caballeria J, Pajares MA, Andreu H, et al. S-adenosyl-l-methionine treatment prevents carbon tetrachloride–induced S-adenosylmethionine synthetase inactivation and attenuates liver injury. Hepatology. 1992;16:1022–1027
  5. DeLeve LD, Kaplowitz N. Glutathione metabolism and its role in hepatotoxicity. Pharmacol Ther. 1991;2:287–305
  6. Feo F, Pascale R, Garcea R, Daino L, Pirisi L, Frassetto S, et al. Effect of the variations of S-adenosyl-l-methionine liver content on fat accumulation and ethanol metabolism in ethanol-intoxicated rats. Toxicol Appl Pharmacol. 1986;83:331–341
  7. Fernández-Checa JC, Garcı́a-Ruiz C, Colell A. Mitochondria in alcoholic liver disease. In:  Lemasters JJ,  Nieminen AL editor. Mitochondria in Pathogenesis. New York: Kluwer Academic Press; 2000;p. 361–377
  8. Fernández-Checa JC, Garcı́a-Ruiz C, Ookhtens M, Kaplowitz N. Impaired uptake of glutathione by hepatic mitochondria from chronic ethanol-fed rats. Tracer kinetic studies in vitro and in vivo and susceptibility to oxidant stress. J Clin Invest. 1991;87:397–405
  9. Fernández-Checa JC, Hirano T, Tsukamoto H, Kaplowitz N. Mitochondrial glutathione depletion in alcoholic liver disease. Alcohol. 1993;10:469–475
  10. Fernández-Checa JC, Kaplowitz N, Garcı́a-Ruiz C, Colell A. Mitochondrial glutathione (importance and transport). Semin Liver Dis. 1998;16:389–401
  11. Fernández-Checa JC, Ookhtens M, Kaplowitz N. Effects of chronic ethanol feeding on rat hepatocytic glutathione. Relationship of cytosolic glutathione to efflux and mitochondrial sequestration. J Clin Invest. 1989;83:1247–1252
  12. Garcı́a-Ruiz C, Morales A, Ballesta A, Rodes J, Kaplowitz N, Fernández-Checa JC. Effect of chronic ethanol feeding on glutathione and functional integrity of mitochondria in periportal and perivenous rat hepatocytes. J Clin Invest. 1994;94:193–201
  13. Garcı́a-Ruiz C, Morales A, Colell A, Ballesta A, Rodes J, Kaplowitz N, et al. Feeding S-adenosyl-l-methionine attenuates both ethanol-induced depletion of mitochondrial glutathione and mitochondrial dysfunction in periportal and perivenous rat hepatocytes. Hepatology. 1995;21:207–214
  14. Hirano T, Kaplowitz N, Tsukamoto H, Kamimura S, Fernández-Checa JC. Hepatic mitochondrial glutathione depletion and progression of experimental alcoholic liver disease in rats. Hepatology. 1992;16:1423–1427
  15. Iimuro Y, Gallucci RM, Luster MI, Kono H, Thurman RG. Antibodies to tumor necrosis factor alfa attenuate hepatic necrosis and inflammation caused by chronic exposure to ethanol in the rat. Hepatology. 1997;26:1530–1537
  16. Jewell SA, Di Monte D, Gentile A, Guglielmi A, Altomare E, Albano O. Decreased hepatic glutathione in chronic alcoholic patients. J Hepatol. 1986;3:1–6
  17. Kato S, Kawase T, Alderman J, Inatomi N, Lieber CS. Role of xanthine oxidase in ethanol-induced lipid peroxidation in rats. Gastroenterology. 1990;98:203–210
  18. Knecht KT, Bradford BU, Mason RP, Thurman RG. In vivo formation of a free radical metabolite of ethanol. Mol Pharmacol. 1990;38:26–30
  19. Kukielka E, Dicker E, Cederbaum AI. Increased production of reactive oxygen species by rat liver mitochondria after chronic ethanol treatment. Arch Biochem Biophys. 1994;309:377–386
  20. Lieber CS, Casini A, DeCarli LM, Kim CI, Lowe N, Sasaki R, et al. S-adenosyl-l-methionine attenuates alcohol-induced liver injury in the baboon. Hepatology. 1990;11:165–172
  21. Lu SC, Huang ZZ, Yang JM, Tsukamoto H. Effect of ethanol and high-fat feeding on hepatic γ-glutamylcysteine synthetase subunit expression in the rat. Hepatology. 1999;30:209–214
  22. Martı́n-Duce A, Ortiz P, Cabrero C, Mato JM. S-adenosyl-l-methionine synthetase and phospholipid methyltransferase are inhibited in human cirrhosis. Hepatology. 1988;8:65–68
  23. Masini A, Ceccarelli D, Galléis D, Giovannini F, Trenti T. Lipid hydroperoxide induced mitochondrial dysfunction following acute ethanol intoxication in rats. The critical role for mitochondrial reduced glutathione. Biochem Pharmacol. 1994;47:217–224
  24. Mato JM, Camara J, Fernandez de Paz J, Caballeria L, Coll S, Caballero A, et al. S-adenosylmethionine in alcoholic liver cirrhosis (a randomized, placebo-controlled, double-blind, multicenter clinical trial). J Hepatol. 1999;30:1081–1089
  25. Misra UK, Bradford BU, Handler JA, Thurman RG. Chronic ethanol treatment induces hydrogen peroxide production selectively in pericentral regions of the liver lobule. Alcohol Clin Exp Res. 1992;16:839–842
  26. Reinke LA, Kotake Y, McCay PB, Janzen EG. Spin-trapping studies of hepatic free radicals formed following the acute administration of ethanol to rats (in vivo detection of 1-hydroxylethyl radicals with PBN). Free Radic Biol Med. 1991;11:31–39
  27. Shaw S, Jayatilleke E, Ross WA, Gordon ER, Lieber CS. Ethanol-induced lipid peroxidation (potentiation by long-term alcohol feeding and attenuation by methionine). J Lab Clin Med. 1981;98:417–424
  28. Shaw S, Rubin KP, Lieber CS. Depressed hepatic glutathione and increased diene conjugates in alcoholic liver disease. Evidence of lipid peroxidation. Dig Dis Sci. 1983;28:585–589
  29. Stramentinoli G, Di Padova C, Gualano M, Rovagnati P, Galli-Kienle M. Ethynylestradiol-induced impairment of bile secretion in the rat (protective effects of S-adenosyl-l-methionine and its implication in estrogen metabolism). Gastroenterology. 1981;80:154–158
  30. Tsukamoto H, Lu SC. Current concepts in the pathogenesis of alcoholic liver injury. FASEB J. 2001;15:1335–1349
  31. Vendemiale G, Altomare E, Grattagliano I, Albano O. Increased plasma levels of glutathione and malondialdehyde after acute ethanol ingestion in humans. J Hepatol. 1989;9:359–365 a
  32. Vendemiale G, Altomare E, Trizio T, Le Grazie C, Di Padova C, Salerno MT, et al. Effects of oral S-adenosyl-l-methionine on hepatic glutathione in patients with liver disease. Scand J Gastroenterol. 1989;18:307–318 b
  33. Videla LA, Iturriaga H, Pino ME, Bunout D, Valenzuela A, Ugarte G. Content of hepatic reduced glutathione in chronic alcoholic patients (influence of the length of abstinence and liver necrosis). Clin Sci. 1984;66:283–290
  34. Wheeler MD, Nakagami M, Bradford BU, Uesugi T, Mason RP, Connor HD, et al. Overexpression of manganese superoxide dismutase prevents alcohol-induced liver injury in the rat. J Biol Chem. 2001;276:36664–36672
  35. Yin M, Wheeler MD, Kono H, Bradford BU, Gallucci RM, Luster MI, et al. Essential role of tumor necrosis factor alpha in alcohol-induced liver injury in mice. Gastroenterology. 1999;117:942–952

☆ Editor: T.R. Jerrells

PII: S0741-8329(02)00229-X

Alcohol
Volume 27, Issue 3 , Pages 179-183 , July 2002

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