Alcohol
Volume 35, Issue 1 , Pages 75-82 , January 2005

Metabolic effects of ethanol on primary cell cultures of rat skeletal muscle

Received 1 May 2004 ,Revised 27 December 2004 ,Accepted 27 December 2004.

References 

  1. Beutler E. Red Cell Metabolism: A Manual of Biochemical Methods. 2nd ed.. New York: Grune & Stratton; 1975;
  2. Beutler HO, Michal G. Neue Methode zur enzymattischen Bestimmung von Äthanol in Lebensmitteln. Z Anal Chem. 1977;284:113–117
  3. Cadefau JA, Parra J, Cussó R, Heine G, Pette D. Responses of fatigable and fatigue-resistant fibres of rabbit muscle to low-frequency stimulation. Pflügers Arch. 1993;424:529–537
  4. Cadefau JA, Parra J, Tauler A, Cussó R. Contractile activity modifies Fru-2,6-P2 metabolism in rabbit fast twitch skeletal muscle. J Biol Chem. 1999;274:31961–31966
  5. Cardellach F, Taraschi TF, Ellingson JS, Stubbs CD, Rubin E, Hoek JB. Maintenance of structural and functional characteristics of skeletal-muscle mitochondria and sarcoplasmic-reticular membranes after chronic ethanol treatment. Biochem J. 1991;274(Pt 2):565–573
  6. Cussó R, Vernet M, Cadefau J, Urbano-Márquez A. Effects of ethanol and acetaldehyde on the enzymes of glycogen metabolism. Alcohol Alcohol. 1989;24:291–297
  7. Davidson AL, Arion WJ. Factors underlying significant underestimations of glucokinase activity in crude liver extracts: physiological implications of higher cellular activity. Arch Biochem Biophys. 1987;253:156–167
  8. Dornhorst A, Ouyang A. Effect of alcohol on glucose tolerance. Lancet. 1971;2:957–959
  9. Ferreira LDMC-B, Palmer TN, Fournier PA. Prolonged exposure to halothane and associated changes in carbohydrate metabolism in rat muscles in vivo. J Appl Physiol. 1998;84:1470–1474
  10. Garriga J, Adanero E, Fernández-Solá J, Urbano-Márquez A, Cussó R. Ethanol inhibits skeletal muscle cell proliferation and delays its differentiation in cell culture. Alcohol Alcohol. 2000;35:236–241
  11. Gilboe DP, Larson KL, Nuttall FQ. Radioactive method for the assay of glycogen phosphorylases. Anal Biochem. 1972;47:20–27
  12. Hayashi T, Wojtaszewski JFP, Goodyear LJ. Exercise regulation of glucose transport in skeletal muscle. Am J Physiol Endocrinol Metab. 1997;273(6 Pt 1):E1039–E1051
  13. Hu IC, Singh SP, Snyder AK. Effects of ethanol on glucose transporter expression in culture hippocampal neurons. Alcohol Clin Exp Res. 1995;19:1398–1402
  14. Institute of Laboratory Animal Resources , Commission on Life Sciences , National Research Council . Guide for the Care and Use of Laboratory Animals. Washington, DC: National Academy Press; 1996;
  15. Labarca C, Paigen K. A simple, rapid, and sensitive DNA assay procedure. Anal Biochem. 1980;102:344–352
  16. Martin FC, Levi AJ, Slavin G, Peters TJ. Glycogen content and activities of key glycolytic enzymes in muscle biopsies from control subjects and patients with chronic alcoholic skeletal myopathy. Clin Sci (Lond). 1984;66:69–78
  17. McCall D, Ryan K. The effect of ethanol and acetaldehyde on Na pump function in cultured rat heart cells. J Mol Cell Cardiol. 1987;19:453–463
  18. Montell E, Turini M, Marotta M, Roberts M, Noé V, Ciudad CJ, et al. DAG accumulation from saturated fatty acids desensitizes insulin stimulus of glucose uptake in muscle cells. Am J Physiol Endocrinol Metab. 2001;280:E229–E237
  19. Narabayashi H, Lawson JWR, Uyeda K. Regulation of phosphofructokinase in perfused rat heart. Requirement for fructose 2,6-bisphosphate and a covalent modification. J Biol Chem. 1985;260:9750–9758
  20. Ohnishi ST, Waring AJ, Fang SR, Horiuchi K, Ohnishi T. Sarcoplasmic reticulum membrane of rat skeletal muscle is disordered with chronic alcohol ingestion. Membr Biochem. 1985;6:49–63
  21. Passonneau JV, Lauderdale VR. A comparison of three methods of glycogen measurement in tissues. Anal Biochem. 1974;60:405–412
  22. Passonneau JV, Lowry OH, Schulz DW, Brown JG. Glucose 1,6-diphosphate formation by phosphoglucomutase in mammalian tissues. J Biol Chem. 1969;244:902–909
  23. Perkoff GT. Alcoholic myopathy. Annu Rev Med. 1971;22:125–132
  24. Shelmet JJ, Reichard GA, Skutches CL, Hoeldtke RD, Owen OE, Boden G. Ethanol causes acute inhibition of carbohydrate, fat, and protein oxidation and insulin resistance. J Clin Invest. 1988;81:1137–1145
  25. Song SK, Rubin E. Ethanol produces muscle damage in human volunteers. Science. 1972;175:327–328
  26. Stephens JM, Pilch PF. The metabolic regulation and vesicular transport of GLUT4, the major insulin-responsive glucose transporter. Endocr Rev. 1995;16:529–546
  27. Thomas JA, Schlender KK, Larner J. A rapid filter paper assay for UDPglucose-glycogen glucosyltransferase, including an improved biosynthesis of UDP-14C-glucose. Anal Biochem. 1968;25:486–499
  28. Urbano-Márquez A, Estruch R, Grau JM, Vernet M, Casademont J. Skeletal muscle changes in chronic alcoholic patients. Acta Neurol Scand. 1985;72:72–73
  29. Urbano-Márquez A, Estruch R, Navarro-Lopez F, Grau JM, Mont L, Rubin E. The effects of alcoholism on skeletal and cardiac muscle. N Engl J Med. 1989;320:409–415
  30. Vandoolaeghe P, Gueuning M-A, Rousseau GG. M-type 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase is the product of a late muscle differentiation gene. Biochem Biophys Res Commun. 1999;259:250–254
  31. Van Schaftingen E, Bartrons R, Hers HG. The mechanism by which ethanol decreases the concentration of fructose 2,6-bisphosphate in the liver. Biochem J. 1984;222:511–518
  32. Van Schaftingen E, Lederer B, Bartrons R, Hers HG. A kinetic study of pyrophosphate: fructose-6-phosphate phosphotransferase from potato tubers. Application to a microassay of fructose 2,6-bisphosphate. Eur J Biochem. 1982;129:191–195
  33. Vernet M, Cadefau JA, Balagué A, Grau JM, Urbano-Márquez A, Cussó R. Effect of chronic alcoholism on human muscle glycogen and glucose metabolism. Alcohol Clin Exp Res. 1995;19:1295–1299
  34. Wilkes JJ, Nagy LE. Chronic ethanol feeding impairs glucose tolerance but does not produce skeletal muscle insulin resistance in rat epitrochlearis muscle. Alcohol Clin Exp Res. 1996;20:1016–1022
  35. Yki-Järvinen H, Nikkila EA. Ethanol decreases glucose utilization in healthy man. J Clin Endocrinol Metab. 1985;61:941–945
  36. Yu B, Schroeder A, Nagy LE. Ethanol stimulates glucose uptake and translocation of GLUT-4 in H9c2 myotubes via a Ca2+-dependent mechanism. Am J Physiol Endocrinol Metab. 2000;279:E1358–E1365

PII: S0741-8329(05)00057-1

doi: 10.1016/j.alcohol.2004.12.003

Alcohol
Volume 35, Issue 1 , Pages 75-82 , January 2005

Article Tools

* Image Usage & Resolution