Alcohol
Volume 35, Issue 1 , Pages 57-66 , January 2005

Formation of acetaldehyde adducts of glutathione S-transferase A3 in the liver of rats administered alcohol chronically

  • Rukhsana Sultana

      Affiliations

    • Department of Animal Sciences, School of Life Sciences, University of Hyderabad, Hyderabad - 500 046, India
    • Present address: Department of Chemistry, University of Kentucky, Lexington, KY 40506, USA.
    • ,
  • Bhupanapadu Sunkesula Solomon Raju

      Affiliations

    • Department of Animal Sciences, School of Life Sciences, University of Hyderabad, Hyderabad - 500 046, India
    • ,
  • Varsha Sharma

      Affiliations

    • School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067
    • ,
  • Pallu Reddanna

      Affiliations

    • Department of Animal Sciences, School of Life Sciences, University of Hyderabad, Hyderabad - 500 046, India
    • ,
  • Phanithi Prakash Babu

      Affiliations

    • Department of Animal Sciences, School of Life Sciences, University of Hyderabad, Hyderabad - 500 046, India
    • Corresponding Author InformationCorresponding author. Tel.: +91-40-23134584; fax: +91-40-23010145/23010120.

Received 23 April 2004 ,Revised 26 November 2004 ,Accepted 4 December 2004.

References 

  1. Alin P, Jensson H, Guthenberg C, Danielson UH, Tahir MK, Mannervik B. Purification of major basic glutathione transferase isoenzymes from rat liver by use of affinity chromatography and fast protein liquid chromatofocusing. Anal Biochem. 1985;146:313–320
  2. Awasthi YC, Dao DD, Saneto RP. Interrelationship between anionic and cationic forms of glutathione S-transferases of human liver. Biochem J. 1980;191:1–10
  3. Babu PP, Kumari LR, Vemuri MC. Differential changes in cell morphology, macromolecular composition and membrane protein profiles of neurons and astrocytes in chronic ethanol treated rats. Mol Cell Biochem. 1994;130:29–40
  4. Baron J, Kawabata T, Knapp S, Voigt JM, Redick JA, Jacoby WB, et al. Intrahepatic distribution of xenobiotic metabolizing enzymes. In:  Caldwell J,  Paulson GD editor. Foreign Compound Metabolism. London: Taylor & Francis; 1984;p. 17–36
  5. Barry RE, Williams AJ, McGivan JD. The detection of acetaldehyde/liver plasma membrane protein adduct formed in vivo by alcohol feeding. Liver. 1987;7:364–368
  6. Board PG, Baker RT, Chelvanayagam G, Jermiin LS. Zeta, a novel class of glutathione transferases in a range of species from plants to humans. Biochem J. 1997;328:929–935
  7. Cederbaum AI. Role of lipid peroxidation and oxidative stress in alcohol toxicity. Free Radic Biol Med. 1989;7:537–539
  8. Cheng J-Z, Singhal SS, Sharma A, Saini M, Yang Y, Awasthi S, et al. Transfection of mGSTA4 in HL-60 cells protects against 4-hydroxynonenal-induced apoptosis by inhibiting JNK-mediated signaling. Arch Biochem Biophys. 2001;392:197–207
  9. Committee for the Purpose of Control and Supervision on Experiments on Animals. (2003). CPCSEA guidelines for laboratory animal facility. Indian Journal of Pharmacology 35, 257–274. Available at: http://icmr.nic.in/bioethics/CPCSEA%20guidelines%20for%20Laboratory%20 Animal%20Facilities.pdf.
  10. Donohue TM, Tuma DJ, Sorrell MF. Acetaldehyde adducts with proteins: binding of [14C]acetaldehyde to serum albumin. Arch Biochem Biophys. 1983;220:239–246
  11. Glassman EB, McLaughlin GA, Forman DT, Felder MR, Thurman RG. Role of alcohol dehydrogenase in the swift increase in alcohol metabolism (SIAM). Studies with deer mice deficient in alcohol dehydrogenase. Biochem Pharmacol. 1985;34:3523–3526
  12. Habig WH, Jakoby WB. Assays for differentiation of glutathione S-transferases. Methods Enzymol. 1981;77:398–405
  13. Hayes JD, Pulford DJ. The glutathione S-transferase supergene family: regulation of GST and the contribution of the isoenzymes to cancer chemoprotection and drug resistance. Crit Rev Biochem Mol Biol. 1995;30:445–600
  14. Israel Y, Hurwitz E, Niemelä O, Arnon R. Monoclonal and polyclonal antibodies against acetaldehyde-containing epitopes in acetaldehyde–protein adducts. Proc Natl Acad Sci U S A. 1986;83:7923–7927
  15. Kesaniemi YA, Kervinen K, Miettinen TA. Acetaldehyde modification of low density lipoprotein accelerates its catabolism in man. Eur J Clin Invest. 1987;17:29–36
  16. Kim CN, Wang X, Huang Y, Ibrado AM, Liu L, Fang G, et al. Overexpression of Bcl-XL inhibits Ara-C-induced mitochondrial loss of cytochrome c and other perturbations that activate the molecular cascade of apoptosis. Cancer Res. 1997;57:3115–3120
  17. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970;227:680–685
  18. Lieber CS. Metabolic effects of acetaldehyde. Biochem Soc Trans. 1988;16:241–247
  19. Lieber CS, DeCarli LM. Ethanol oxidation by hepatic microsomes: adaptive increase after ethanol feeding. Science. 1968;162:917–918
  20. Lin RC, Smith RS, Lumeng L. Detection of a protein–acetaldehyde adduct in the liver of rats fed alcohol chronically. J Clin Invest. 1988;81:615–619
  21. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951;193:265–275
  22. Mannervik B, Alin P, Guthenberg C, Jensson H, Tahir MK, Warholm M, et al. Identification of three classes of cytosolic glutathione transferase common to several mammalian species: correlation between structural data and enzymatic properties. Proc Natl Acad Sci U S A. 1985;82:7202–7206
  23. Mannervik B, Danielson UH. Glutathione transferases—structure and catalytic activity. CRC Crit Rev Biochem. 1988;23:283–337
  24. Mannervik B, Guthenberg C, Jensson H, Warholm M, Alin P. Isozymes of glutathione S-transferase in rat and human tissues. In:  Larsson A editors. Functions of Glutathione: Biochemical, Physiological, Toxicological, and Clinical Aspects. New York: Raven Press; 1983;p. 75–88
  25. Meyer DJ, Coles B, Pemble SE, Gilmore KS, Fraser GM, Ketterer B. Theta, a new class of glutathione transferases purified from rat and man. Biochem J. 1991;274(Pt 2):409–414
  26. Niemelä O. Aldehyde–protein adducts in the liver as a result of ethanol-induced oxidative stress. Front Biosci. 1999;4:D506–D513
  27. Niemelä O, Parkkila S, Yla-Herttuala S, Villanueva J, Ruebner B, Halsted CH. Sequential acetaldehyde production, lipid peroxidation, and fibrogenesis in micropig model of alcohol-induced liver disease. Hepatology. 1995;22(4 Pt 1):1208–1214
  28. Pemble SE, Wardle AF, Taylor JB. Glutathione S-transferase class Kappa: characterization by the cloning of rat mitochondrial GST and identification of a human homologue. Biochem J. 1996;319:749–754
  29. Peterson CM, Polizzi CM. Improved method for acetaldehyde in plasma and hemoglobin-associated acetaldehyde: results in teetotalers and alcoholics reporting for treatment. Alcohol. 1987;4:477–480
  30. Simons PC, Vander Jagt DL. Purification of glutathione S-transferases from human liver by glutathione-affinity chromatography. Anal Biochem. 1977;82:334–341
  31. Singhal SS, Zimniak P, Awasthi S, Piper JT, He N-g, Teng JI, et al. Several closely related glutathione S-transferase isozymes catalyzing conjugation of 4-hydroxynonenal are differentially expressed in human tissues. Arch Biochem Biophys. 1994;311:242–250
  32. Stenberg G, Ridderstrom M, Engstrom A, Pemble SE, Mannervik B. Cloning and heterologous expression of cDNA encoding class alpha rat glutathione transferase 8-8, an enzyme with high catalytic activity towards genotoxic α,β-unsaturated carbonyl compounds. Biochem J. 1992;284(Pt 2):313–319
  33. Stevens VJ, Fantl WJ, Newman CB, Sims RV, Cerami A, Peterson CM. Acetaldehyde adducts with hemoglobin. J Clin Invest. 1981;67:361–369
  34. Tietjen TG, Mjaatvedt CH, Yang VW. Cellular localization of the class I alcohol dehydrogenase transcript in adult rat tissues. Histochem J. 1994;26:526–532
  35. Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979;76:4350–4354
  36. Tuma DJ, Sorrell MF. The role of acetaldehyde adducts in liver injury. In:  Hall P editors. Alcoholic Liver Disease: Pathology and Pathogenesis. 2nd ed.. London: Edward Arnold; 1995;p. 89–99
  37. Vandenberghe Y, Glaise D, Meyer DJ, Guillouzo A, Ketterer B. Glutathione transferase isoenzymes in cultured rat hepatocytes. Biochem Pharmacol. 1988;37:2482–2485
  38. Vanhaecke T, Vercruysse A, Rogiers V. Effect of pyruvate on glutathione S-transferase expression in primary cultures of rat hepatocytes. Toxicol In Vitro. 1997;11:435–441

PII: S0741-8329(05)00058-3

doi: 10.1016/j.alcohol.2004.12.004

Alcohol
Volume 35, Issue 1 , Pages 57-66 , January 2005

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